Please use this identifier to cite or link to this item: https://repository.usc.edu.co/handle/20.500.12421/479
Title: The role of the peritrophic matrix and red blood cell concentration in Plasmodium vivax infection of Anopheles aquasalis.
Authors: Baia-da-Silva, Djane Clarys
Salazar Alvarez, Luis Carlos
Vera Lizcano, Omaira
Maranhão Costa, Fabio Trindade
Costa Pinto Lopes, Stefanie
Silva Orfanó, Alessandra
Oliveira Pascoal, Denner
Nacif-Pimenta, Rafael
Cabral Rodriguez, Iria
Barbosa Guerra, Maria das Graças Vale
Guimarães Lacerda, Marcus Vinicius
Costa Secundino, Nagila Francinete
Monteiro, Wuelton Marcelo
Paolucci Pimenta, Paulo Filemon
Keywords: Malaria;Plasmodium vivax;Peritrophic matrix;Trypsin;Chitinase;Hematocrit
Issue Date: 2018
Publisher: Parasites and Vectors
Abstract: Background: Plasmodium vivax is predominant in the Amazon region, and enhanced knowledge of its development inside a natural vector, Anopheles aquasalis, is critical for future strategies aimed at blocking parasite development. The peritrophic matrix (PM), a chitinous layer produced by the mosquito midgut in response to blood ingestion, is a protective barrier against pathogens. Plasmodium can only complete its life-cycle, and consequently be transmitted to a new host, after successfully passing this barrier. Interestingly, fully engorged mosquitoes that had a complete blood meal form a thicker, well-developed PM than ones that feed in small amounts. The amount of red blood cells (RBC) in the blood meal directly influences the production of digestive enzymes and can protect parasites from being killed during the meal digestion. A specific study interrupting the development of the PM associated with the proteolytic activity inhibition, and distinct RBC concentrations, during the P. vivax infection of the New World malaria vector An. aquasalis is expected to clarify whether these factors affect the parasite development. Results: Absence of PM in the vector caused a significant reduction in P. vivax infection. However, the association of chitinase with trypsin inhibitor restored infection rates to those of mosquitoes with a structured PM. Also, only the ingestion of trypsin inhibitor by non-chitinase treated mosquitoes increased the infection intensity. Moreover, the RBC concentration in the infected P. vivax blood meal directly influenced the infection rate and its intensity. A straight correlation was observed between RBC concentrations and infection intensity. Conclusions: This study established that there is a balance between the PM role, RBC concentration and digestive enzyme activity influencing the establishment and development of P. vivax infection inside An. aquasalis. Our results indicate that the absence of PM in the midgut facilitates digestive enzyme dispersion throughout the blood meal, causing direct damage to P. vivax. On the other hand, high RBC concentrations support a better and thick, well-developed PM and protect P. vivax from being killed. Further studies of this complex system may provide insights into other details of the malaria vector response to P. vivax infection.
URI: https://repository.usc.edu.co/handle/20.500.12421/479
ISSN: 1756-3305
Appears in Collections:Producción Científica

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