Please use this identifier to cite or link to this item: https://repository.usc.edu.co/handle/20.500.12421/304
Title: Search of allosteric inhibitors and associated proteins of an AKT-like kinase from trypanosoma cruzi
Authors: Ochoa, Rodrigo
Rocha-Roa, Cristian
Marín-Villa, Marcel
Robledo, Sara María
Varela-M, R. E.
Keywords: Allosteric Regulation
Animals
Ligands
Models, Molecular
Parasites
Protein Interaction Maps
Protein Interaction Maps
Proto-Oncogene Proteins c-akt
Protozoan Proteins
Risk Factors
Trypanosoma cruzi
Drug discovery
Pleckstrin homology domain
Protein kinase B
Trypanosoma cruzi
doxorubicin
validation stud
nuclear magnetic resonance spectroscopy
Issue Date: 12-Dec-2018
Publisher: MDPI AG
Abstract: Proteins associated to the PI3K/AKT/mTOR signaling pathway are widely used targets for cancer treatment, and in recent years they have also been evaluated as putative targets in trypanosomatids parasites, such as Trypanosoma cruzi. Here, we performed a virtual screening approach to find candidates that can bind regions on or near the Pleckstrin homology domain of an AKT-like protein in T. cruzi. The compounds were also evaluated in vitro. The in silico and experimental results allowed us to identify a set of compounds that can potentially alter the intracellular signaling pathway through the AKT-like kinase of the parasite; among them, a derivative of the pyrazolopyridine nucleus with an IC 50 of 14.25 ± 1.00 µM against amastigotes of T. cruzi. In addition, we built a protein–protein interaction network of T. cruzi to understand the role of the AKT-like protein in the parasite, and look for additional proteins that can be postulated as possible novel molecular targets for the rational design of compounds against T. cruzi. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.
URI: https://repository.usc.edu.co/handle/20.500.12421/304
ISSN: 16616596
Appears in Collections:Artículos Científicos



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