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|Title:||The oleaginous yeast Meyerozyma guilliermondii BI281A as a new potential biodiesel feedstock: Selection and lipid production optimization|
Jaramillo-García, Victoria P.
Rosa, Priscila D.
Landell, Melissa Fontes
Fabricio, Mariana F.
Ayub, Marco Antônio Záchia
Robert, Vincent A.R.G.
Henriques, João Antonio Pêgas
|Keywords:||Biodiesel;Fatty acids;Lipid production;Nile Red;Raw glycerol;Critical micelle concentration;Meyerozyma guilliermondii;Gas chromatography;Optical density;Nonhuman;Lipogenesis|
|Publisher:||Frontiers Media S.A.|
|Abstract:||A high throughput screening (HTS) methodology for evaluation of cellular lipid content based on Nile red fluorescence reads using black background 96-wells test plates and a plate reader equipment allowed the rapid intracellular lipid estimation of strains from a Brazilian phylloplane yeast collection. A new oleaginous yeast, Meyerozyma guilliermondii BI281A, was selected, for which the gravimetric determination of total lipids relative to dry weight was 52.38% for glucose or 34.97% for pure glycerol. The lipid production was optimized obtaining 108 mg/L of neutral lipids using pure glycerol as carbon source, and the strain proved capable of accumulating oil using raw glycerol from a biodiesel refinery. The lipid profile showed monounsaturated fatty acids (MUFA) varying between 56 or 74% in pure or raw glycerol, respectively. M. guilliermondii BI281A bears potential as a new biodiesel feedstock. © 2017 Ramírez-Castrillón, Jaramillo-Garcia, Rosa, Landell, Vu, Fabricio, Ayub, Robert, Henriques and Valente.|
|Appears in Collections:||Artículos Científicos|
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