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Title: Development of a novel formulation with hypericin to treat cutaneous leishmaniasis based on photodynamic therapy in in vitro and in vivo studies
Authors: Montoya, Andrés
Daza, Alejandro
Muñoz, Diana
Ríos, Karina
Taylor, Viviana
Cedeño, David
Vélez, Iván D.
Echeverri, Fernando
Robledoa, Sara M.
Keywords: Animals;Cell Line;Cricetinae;Humans;Leishmania;Leishmaniasis, Cutaneous;Perylene;Photochemotherapy;Photosensitizing Agents;U937 Cells;Wound Healing;drug absorption;drug efficacy;cytotoxicity;amphotericin B deoxycholate;epidermal growth factor;glyceraldehyde 3 phosphate dehydrogenase
Issue Date: 1-Sep-2015
Publisher: Universidad Santiago de Cali
Abstract: An evaluation of the leishmanicidal activity in vitro and in vivo of hypericin, an expanded-spectrum photosensitizer found in Hypericum perforatum, is presented. Hypericin was evaluated against intracellular amastigotes in vitro of Leishmania (Viannia) panamensis. A topical formulation containing 0.5% hypericin was developed and assayed in vivo in a hamster model of cutaneous leishmaniasis. Results demonstrate that hypericin induces a significant antiamastigote effect in vitro against L. panamensis by decreasing the number of parasites inside infected cells. The topical formulation of 0.5% hypericin allows healing of L. panamensis-induced lesions upon a topical application of 40 mg/day plus visible-light irradiation (5 J/cm 2 , 15 min), twice a week for 3 weeks. Copyright © 2015, American Society for Microbiology. All Rights Reserved.
ISSN: 00664804
Appears in Collections:Artículos Científicos

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